PCR & Sanger sequencing
The course will provide you the basic concepts of the Polymerase Chain Reaction (PCR) and DNA sequencing with the chain termination method (Sanger sequencing). You will learn how to prepare PCR and sequencing reactions without contaminations. The course also addresses the design of PCR and Sanger sequencing protocols and how to analyse the results.
COURSE TOPICS
Polymerase Chain Reaction (PCR)
Sanger sequencing
Designing PCR primers
Setting up reactions
Avoiding contaminations
Equipment and reagents
Laboratory procedures
Data analyses
PARTICIPANTS
The training course are suitable for a wide range of professionals employed in public or private laboratories working in research or customer services. For example:
Laboratory Managers
Scientific Personnel
Laboratory Technicians
Newly recruited staff
ACQUIRED COMPETENCIES
Design a PCR and DNA sequencing protocol
Setting up reactions
Avoiding contaminations
Equipment handling
Interpreting PCR and sequencing results
Data analyses
Basic concepts on molecular biology
TRAINING METHODOLOGY
The courses include lectures and laboratory sessions. The training includes presentations with instructor-guided interactive discussions with participants. The practical lessons are designed to allow all participants to train extensively the teaching procedures. We provide forms, protocols and additional resources for future use within your laboratory.
INSTRUCTORS
The courses are conducted by specialized scientists with vast experience in the lectured topics.
CERTIFICATES
All participants receive a certificate of attendance and a record detailing the topics covered during the course.
TEACHING AND EVALUATION LANGUAGES
English or Portuguese.
