Metabarcoding of Fungal Communities

Uncover the fungal communities in your sample via Metabarcoding (also known as “Amplicon sequencing”).
Send us your sample (cultures, tissues, soil, food, etc.) and we will extract the DNA using our robust high-throughput methods. The sample DNA is then amplified by PCR for an informative genetic marker and analysed by massive parallel sequencing (next-generation sequencing).
You receive a detailed report describing the species composition, diversity indexes, evolutionary analysis, among others.

SKU: META002 Category:

Our services
Sample collection
DNA extraction
Sequencing
Bioinformatics
Report/manuscript preparation

Applications
Identification of species
Detection of contaminants
Analyses of mixtures
Environmental remediation
Scat and Diet Analysis
Gut microbe characterization
Phylogenetic reconstructions
Ecological surveys
Crop management
Biotechnology
… and many others

Sample types
Pure cultures
Soil
Faeces
Water
Food matrices
Biofilms
(a minimum amount of material will be requested depending on the sample characteristics)

Genetic markers
18S rRNA
ITS1
ITS2
(other targets can be analysed upon request)

Sequencing technology
Amplicon sequencing in an Illumina NovaSeq 6000.
30K to 100K raw tags

Bioinformatics
Species annotation
Operational Taxonomic Units (OTUs)
Composition of communities
Alpha / Beta diversity
Evolutionary trees
(and others)

For Research Use Only. Not for use in the diagnosis, prevention, or treatment of a disease.